Cell Line Development

Developing High Quality, High Titre Manufacturing Cell Lines with CHOBC®

Polpharma Biologics have over 25 years of success in generating high-yield production cell lines for innovative proteins.

Our proprietary CHOBC® expression platform allows rapid generation of high-quality cell lines with a completely animal component-free (ACF) process. High-throughput technologies are deployed to increase productivity and achieve the highest titers possible, while minimizing undesired species. We can also rapidly scale to 50 L production for early stage material or transfer to our manufacturing stream for process development and 50 – 2000 L cGMP production.

Our CHOBC® expression platform combines the following features:

  • CHO-K1 derived suspension cell line
  • Allows rapid generation of high-quality cell lines
  • Completely animal component-free (ACF) process
  • Grown in chemically defined media
  • High-throughput technologies are deployed to increase productivity and achieve the highest titers possible, while minimizing undesired species
  • Research cell banks are rigorously tested for the absence of viral elements in accordance with ICH guidelines.
  • CHOBC® cell lines have already been successfully scaled-up to 1,000 L and used to produce drug substance for clinical trial supply.
  • Competitive timelines from DNA to research cell bank
  • Quick scale up to 50 L for pre-clinical supply
  • Combine with our master cell banking, process development and manufacturing services for a fast route to IND

SLIM™ technology improves process efficiency and allows for further process optimization

Our Scientific Leverage of Innovative Metabolism (SLIM) technology reduces feed consumption at much lower power input eliminating hardware limitations in upstream development, reducing the cost of development.

Enhance expression with SPOT™ technology to deliver up to 9 g/L

Our CHOBC® cell line delivers good tires as standard from 2-4 g/L but we have developed our SPOT (Specific Productivity Optimization Technology) which increases cell size and specific expression of the desired protein without increasing the amount of host cell protein or other undesired excipients. This significantly reduces downstream costs due to easier purification.

Specific productivity increases seen once SPOT technology has been applied to CHOBC® cell lines. Titer increases from 150 to 300% are regularly observed.

Accessing CHOBC®, SLIM and SPOT Technology

Polpharma Biologics offer their cell line development packages on a fee for service basis, this can either be as a stand alone cell line development project or as part of an integrated Idea to IND package. We can also work on improving your current cell lines productivity with our SLIM or SPOT technology.

Contact us today for more information or a quote.


Discovery & Optimization

Our early stage antibody discovery and humanization platforms are designed to get you a high affinity antibody with the desired binding properties that is ready for the next stage of your project.


Process Development

Polpharma Biologics provides full process development support, from early development of mAbs to process and analytical methods development.


Analytical Development

We can establish and qualify all required physico-chemical and bioanalytical methods in-house to fully characterize relevant target protein quality attributes.



Our drug substance process development and manufacturing services are fully integrated across our site network for seamless process transfer, scale-up and end-to-end supply chain efficiency.